Development of a Rapid Foodborne-pathogen-detection Method Involving Whole-genome Amplification
نویسندگان
چکیده
منابع مشابه
Rapid molecular techniques for detection of foodborne Bacillus cereus pathogen
Background and Objective: Bacillus cereus is responsible for several outbreaks of foodborne diseases. Therefore, the purpose of the study was to replace routine culture testing, by employing PCR directly from food to detect the bacteria and its enterotoxins. Methods: In the present study, a total of 75 Kibda sandwiches, Sausage sandwiches, Luncheon chicken, Luncheon meat and shawarma sandwiche...
متن کاملGenomePlex Whole-Genome Amplification.
INTRODUCTIONPCR-based whole-genome amplification (WGA) has the goal of generating microgram quantities of genome-representative DNA from picogram or nanogram amounts of starting material. This amplification should introduce little, or ideally no, representational bias. In contrast to other techniques for WGA, PCR-based methods are generally less affected by DNA quality and are more applicable t...
متن کاملDetection of tumor DNA in plasma using whole genome amplification.
Altered microsatellite DNA in the blood of cancer patients may provide a novel means for tumor detection. Such alterations are a major characteristic of many types of tumor especially those associated with head or neck cancer. Moreover, recent evidence suggests that senescent tumor cells release DNA into the circulation, which is subsequently carried by the blood and thus enriched in the serum ...
متن کاملAptamer-Based Technologies in Foodborne Pathogen Detection
Aptamers are single stranded DNA or RNA ligands, which can be selected by a method called systematic evolution of ligands by exponential enrichment (SELEX); and they can specifically recognize and bind to their targets. These unique characteristics of aptamers offer great potentials in applications such as pathogen detection and biomolecular screening. Pathogen detection is the critical means i...
متن کاملMethod for manufacturing whole-genome microarrays by rolling circle amplification.
Comparative genomic hybridization (CGH) to metaphase chromosomes is a method for genome-wide detection of chromosomal aberrations in DNA samples. Recent advances in microarray technology have improved CGH by replacing metaphase chromosomes with a collection of mapped genomic clones placed on glass slides. However, it is quite expensive and labor-intensive to prepare DNA from the genomic clones ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Korean Journal of Food Science and Technology
سال: 2016
ISSN: 0367-6293
DOI: 10.9721/kjfst.2016.48.2.128